Label-free detection of synthetic, full genomic length HIV-1 RNA at the few-copy level.
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ABSTRACT:
SUBMITTER: Dickens OO
PROVIDER: S-EPMC10299804 | biostudies-literature | 2023 Sep
REPOSITORIES: biostudies-literature
Publications
Label-free detection of synthetic, full genomic length HIV-1 RNA at the few-copy level.
Dickens Olivia O OO Bajwa Inayat I Garcia-Ramos Kelly K Suh Yeonjoon Y Wen Chengyu C Cheng Annie A Fethke Victoria V Yi Yanji Y Collman Ronald G RG Johnson A T Charlie ATC
Sensors and actuators. B, Chemical 20230520
Oligonucleotide-functionalized graphene biosensors show immense promise for use as label-free point of care devices for detection of nucleic acid biomarkers at clinically relevant levels. Graphene-based nucleic acid sensors can be fabricated at low cost and have been shown to reach limits of detection in the attomolar range. Here we demonstrate devices functionalized with 22mer or 8omer DNA probes are capable of detecting full length genomic HIV-1 subtype B RNA, with a limit of detection below 1 ...[more]
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