Droplet-based combinatorial indexing for massive-scale single-cell chromatin accessibility.

ABSTRACT: Recent technical advancements have facilitated the mapping of epigenomes at single-cell resolution; however, the throughput and quality of these methods have limited their widespread adoption. Here we describe a high-quality (10⁵ nuclear fragments per cell) droplet-microfluidics-based method for single-cell profiling of chromatin accessibility. We use this approach, named 'droplet single-cell assay for transposase-accessible chromatin using sequencing' (dscATAC-seq), to assay 46,653 cells for the unbiased discovery of cell types and regulatory elements in adult mouse brain. We further increase the throughput of this platform by combining it with combinatorial indexing (dsciATAC-seq), enabling single-cell studies at a massive scale. We demonstrate the utility of this approach by

SUBMITTER: Lareau CA 

PROVIDER: S-EPMC10299900 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature