Colorimetric bacteria sensing of Pseudomonas aeruginosa using gold nanoparticle probes.

ABSTRACT:

Background

Due to the advantages of molecular methods over biochemical methods, the use of molecular methods for diagnosing nosocomial infections such as Pseudomonas can be an appropriate and rapid way to choose the right diagnosis and treatment of infection and prevent further complications caused by the infection. The present article provides a description of the development of a nanoparticle-based detection technique for sensitive and specific deoxyribonucleic acid-based diagnostic of Pseudomonas aeruginosa. Specific thiolated oligonucleotide probes for one of the hypervariable regions of the 16S rDNA gene were designed and applied for colorimetric detection of the bacteria.

Results

The results of gold nanoprobe-nucleic sequence amplification indicated the probe attached

SUBMITTER: Mousivand Z 

PROVIDER: S-EPMC10299975 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature