Application of immuno- and affinity labeling with fluorescent dyes to in-resin CLEM of Epon-embedded cells.

ABSTRACT: In-resin CLEM (Correlative Light and Electron Microscopy) of Epon-embedded cells involves correlating fluorescence microscopy with electron microscopy in the same Epon-embedded ultrathin section. This method offers the advantage of high positional accuracy compared to standard CLEM. However, it requires the expression of recombinant proteins. In order to detect the localization of endogenous target(s) and their localized ultrastructures of Epon-embedded samples using in-resin CLEM, we investigated whether immunological and affinity-labeling using fluorescent dyes applied to in-resin CLEM of Epon-embedded cells. The orange fluorescent (λ_em ∼550 nm) and far-red (λ_em ∼650 nm) fluorescent dyes examined maintained a sufficient level of fluorescent intensity after staining

SUBMITTER: Tanida I 

PROVIDER: S-EPMC10300206 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature