Universal closed-tube barcoding for monitoring the shark and ray trade in megadiverse conservation hotspots.

ABSTRACT: Trade restrictions for endangered elasmobranch species exist to disincentivise their exploitation and curb their declines. However, trade monitoring is challenging due to product variety and the complexity of import/export routes. We investigate the use of a portable, universal, DNA-based tool which would greatly facilitate in-situ monitoring. We collected shark and ray samples across the Island of Java, Indonesia, and selected 28 commonly encountered species (including 22 CITES-listed species) to test a recently developed real-time PCR single-assay originally developed for screening bony fish. In the absence of a bespoke elasmobranch identification online platform in the original FASTFISH-ID model, we employed a deep learning algorithm to recognize species based on DNA melt-curve s

SUBMITTER: Prasetyo AP 

PROVIDER: S-EPMC10300358 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature