Extracellular matrix composition affects outgrowth of dendrites and dendritic spines on cortical neurons.

ABSTRACT: The composition of the extracellular matrix (ECM) in nervous tissue plays an important role in controlling neuronal outgrowth and synapse development. Changes in both protein and glycosaminoglycan components of the ECM occur with tissue injury and may affect neuron growth. To investigate neuron responses to alterations in fibronectin (FN), a major component of the wound ECM, we grew cortical neurons on cell-derived decellularized matrices composed of wild type FN (FN^+/+) or of a mutant form of FN (FN^Δ/+) from which the III₁₃ heparin-binding site had been deleted by CRISPR-Cas 9 gene editing. The most significant effect of the mutant FN was a reduction in dendrite outgrowth. Not only were dendrites shorter on mutant FN^Δ/+-collagen (COL) matrix tha

SUBMITTER: Sharma A 

PROVIDER: S-EPMC10300442 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature