Adventitia-derived extracellular matrix hydrogel enhances contractility of human vasa vasorum-derived pericytes via α₂ β₁ integrin and TGFβ receptor.

ABSTRACT: Pericytes are essential components of small blood vessels and are found in human aortic vasa vasorum. Prior work uncovered lower vasa vasorum density and decreased levels of pro-angiogenic growth factors in adventitial specimens of human ascending thoracic aortic aneurysm. We hypothesized that adventitial extracellular matrix (ECM) from normal aorta promotes pericyte function by increasing pericyte contractile function through mechanisms deficient in ECM derived from aneurysmal aortic adventitia. ECM biomaterials were prepared as lyophilized particulates from decellularized adventitial specimens of human and porcine aorta. Immortalized human aortic adventitia-derived pericytes were cultured within Type I collagen gels in the presence or absence of human or porcine adventitial ECMs. Cell co

SUBMITTER: Wintruba KL 

PROVIDER: S-EPMC10300618 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature