Nuclear mRNA metabolism drives selective basket assembly on a subset of nuclear pore complexes in budding yeast.

ABSTRACT: To determine which transcripts should reach the cytoplasm for translation, eukaryotic cells have established mechanisms to regulate selective mRNA export through the nuclear pore complex (NPC). The nuclear basket, a substructure of the NPC protruding into the nucleoplasm, is thought to function as a stable platform where mRNA-protein complexes (mRNPs) are rearranged and undergo quality control prior to export, ensuring that only mature mRNAs reach the cytoplasm. Here, we use proteomic, genetic, live-cell, and single-molecule resolution microscopy approaches in budding yeast to demonstrate that basket formation is dependent on RNA polymerase II transcription and subsequent mRNP processing. We further show that while all NPCs can bind Mlp1, baskets assemble only on a subset of nucleoplasmic

SUBMITTER: Bensidoun P 

PROVIDER: S-EPMC10300651 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature