Advancing the use of molecularly imprinted polymers in bioanalysis: the selective extraction of cotinine in human urine.

ABSTRACT: Aim: To characterize a molecularly imprinted polymer via precipitation polymerization for the extraction of cotinine in urine. Methods: The polymer was created via precipitation polymerization. Physical characteristics of the polymer were assessed via scanning electron microscopy, Fourier transform infrared spectroscopy and thermogravimetric analysis. The polymer adsorption capacity was assessed and an solid-phase extraction method from urine by LC-MS/MS was developed. Results: The polymer had small, spherical morphology and little thermal decomposition. The extraction method yielded cotinine recoveries of 77-103% in urine. The molecularly imprinted polymer adsorption capacity for cotinine was 448.2 ± 2.1 μg/mg. Common interferants did not affect cotinine's extraction.

SUBMITTER: Mulder HA 

PROVIDER: S-EPMC10300667 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature