A Novel UHPLC-MS/MS Method for the Quantification of Seven Opioids in Different Human Tissues.

ABSTRACT:

Background

Opioids are considered the cornerstone of pain management: they show good efficacy as a first-line therapy for moderate to severe cancer pain. Since pharmacokinetic/pharmacodynamic information about the tissue-specific effect and toxicity of opioids is still scarce, their quantification in post-mortem autoptic specimens could give interesting insights.

Methods

We describe an ultra-high-performance liquid chromatography coupled with tandem mass spectrometry method for the simultaneous quantification of methadone, morphine, oxycodone, hydrocodone, oxymorphone, hydromorphone and fentanyl in several tissues: liver, brain, kidney, abdominal adipose tissue, lung and blood plasma. The presented method has been applied on 28 autoptic samples from different organs obtained

SUBMITTER: Manca A 

PROVIDER: S-EPMC10300969 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature