The Mycobacterium smegmatis HesB Protein, MSMEG_4272, Is Required for In Vitro Growth and Iron Homeostasis.
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SUBMITTER: Niemand Wolhuter N
PROVIDER: S-EPMC10301553 | biostudies-literature | 2023 Jun
REPOSITORIES: biostudies-literature
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The <i>Mycobacterium smegmatis</i> HesB Protein, MSMEG_4272, Is Required for In Vitro Growth and Iron Homeostasis.
Niemand Wolhuter Nandi N Ngakane Lerato L de Wet Timothy J TJ Warren Robin M RM Williams Monique J MJ
Microorganisms 20230614 6
A-type carrier (ATC) proteins are proposed to function in the biogenesis of Fe-S clusters, although their exact role remains controversial. The genome of <i>Mycobacterium smegmatis</i> encodes a single ATC protein, MSMEG_4272, which belongs to the HesB/YadR/YfhF family of proteins. Attempts to generate an <i>MSMEG</i>_<i>4272</i> deletion mutant by two-step allelic exchange were unsuccessful, suggesting that the gene is essential for in vitro growth. CRISPRi-mediated transcriptional knock-down o ...[more]
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