Exploring Proton-Only NMR Experiments and Filters for Daphnia In Vivo: Potential and Limitations.

ABSTRACT: Environmental metabolomics provides insight into how anthropogenic activities have an impact on the health of an organism at the molecular level. Within this field, in vivo NMR stands out as a powerful tool for monitoring real-time changes in an organism's metabolome. Typically, these studies use 2D ¹³C-¹H experiments on ¹³C-enriched organisms. Daphnia are the most studied species, given their widespread use in toxicity testing. However, with COVID-19 and other geopolitical factors, the cost of isotope enrichment increased ~6-7 fold over the last two years, making ¹³C-enriched cultures difficult to maintain. Thus, it is essential to revisit proton-only in vivo NMR and ask, "Can any metabolic information be obtained from Daphnia using proton-only

SUBMITTER: Ronda K 

PROVIDER: S-EPMC10301599 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature