Characterizing conjugative plasmids from an antibiotic-resistant dataset for use as broad-host delivery vectors.

ABSTRACT: Human microbiome engineering is increasingly proposed as a way to modulate health outcomes. However, one of the current limitations to engineering microbial communities in situ is delivery of a genetic payload for introducing or modifying genes. Indeed, there is a need to identify novel broad-host delivery vectors for microbiome engineering. Therefore, in this study, we characterized conjugative plasmids from a publicly available dataset of antibiotic-resistant isolate genomes in order to identify potential broad-host vectors for further applications. From the 199 closed genomes available in the CDC & FDA AR Isolate Bank, we identified 439 plasmids, of which 126 were predicted to be mobilizable and 206 conjugative. Various characteristics of the conjugative plasmids, such as size, r

SUBMITTER: Loyola Irizarry HG 

PROVIDER: S-EPMC10301749 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature