Phase Separation Modulates the Formation and Stabilities of DNA Guanine Quadruplex.

ABSTRACT: In the presence of monovalent alkali metal ions, G-rich DNA sequences containing four runs of contiguous guanines can fold into G-quadruplex (G4) structures. Recent studies showed that these structures are located in critical regions of the human genome and assume important functions in many essential DNA metabolic processes, including replication, transcription, and repair. However, not all potential G4-forming sequences are actually folded into G4 structures in cells, where G4 structures are known to be dynamic and modulated by G4-binding proteins as well as helicases. It remains unclear whether there are other factors influencing the formation and stability of G4 structures in cells. Herein, we showed that DNA G4s can undergo phase separation in vitro. In addition, immunofluoresc

SUBMITTER: Gao Z 

PROVIDER: S-EPMC10301798 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature