A Ratiometric Fluorescence Amplification Using Copper Nanoclusters with o-Phenylenediamine Sensor for Determination of Mercury (II) in Natural Water.

ABSTRACT: A simple and rapid method for determining mercury (II) has been developed using L-cysteine-capped copper nanocluster (CuNCs) with o-phenylenediamine (OPD) as the sensor. The characteristic fluorescence peak of the synthesized CuNCs was observed at 460 nm. The fluorescence properties of CuNCs were strongly influenced by the addition of mercury (II). Upon addition, CuNCs were oxidized to form Cu²⁺. Then, the OPD were rapidly oxidized by Cu²⁺ to form o-phenylenediamine oxide (oxOPD), as evidenced by the strong fluorescence peak at 547 nm, resulting in a decrease in the fluorescence intensity at 460 nm and an increase in the fluorescence intensity at 547 nm. Under optimal conditions, a calibration curve between the fluorescence ratio (I547/I460) and mercury

SUBMITTER: Phoungsiri A 

PROVIDER: S-EPMC10302021 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature