Molecular Mechanism of Nucleosome Recognition by the Pioneer Transcription Factor Sox.

ABSTRACT: Pioneer transcription factors (PTFs) have the remarkable ability to directly bind to chromatin to stimulate vital cellular processes. In this work, we dissect the universal binding mode of Sox PTF by combining extensive molecular simulations and physiochemistry approaches, along with DNA footprinting techniques. As a result, we show that when Sox consensus DNA is located at the solvent-facing DNA strand, Sox binds to the compact nucleosome without imposing any significant conformational changes. We also reveal that the base-specific Sox:DNA interactions (base reading) and Sox-induced DNA changes (shape reading) are concurrently required for sequence-specific nucleosomal DNA recognition. Among three different nucleosome positions located on the positive DNA arm, a sequence-specific reading

SUBMITTER: Ozden B 

PROVIDER: S-EPMC10302475 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature