Optimizing the number of variants tracked to follow disease burden with circulating tumor DNA assays in metastatic colorectal cancer.

ABSTRACT:

Background

The number of somatic mutations detectable in circulating tumor DNA (ctDNA) is highly heterogeneous in metastatic colorectal cancer (mCRC). The optimal number of mutations required to assess disease kinetics is relevant and remains poorly understood.

Objectives

To determine whether increasing panel breadth (the number of tracked variants in a ctDNA assay) would alter the sensitivity in detecting ctDNA in patients with mCRC.

Design

We used archival tissue sequencing to perform an in silico assessment of the optimal number of tracked mutations to detect and monitor disease kinetics in mCRC using sequencing data from the Canadian Cancer Trials Group CO.26 trial.

Methods

For each patient, 1, 2, 4, 8, 12, or 16 of the most clonal (highest variant a

SUBMITTER: Boutin M 

PROVIDER: S-EPMC10302520 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature