Tumor-secreted IFI35 promotes proliferation and cytotoxic activity of CD8⁺ T cells through PI3K/AKT/mTOR signaling pathway in colorectal cancer.

ABSTRACT:

Background

A large proportion of the patients with cancer do not respond to immunotherapies. Recent studies suggested an important role for tumor-infiltrating cytotoxic T lymphocytes (CTL) in enhancing response to immunotherapy. Here, we aim to identify gene that induce proliferative and cytotoxic states of CD8⁺ T cells, and to investigate its effect on CAR-T cells against colorectal cancer.

Methods

Correlation between the expression of IFI35 with the activation and cytotoxicity of CD8⁺ T cells was assessed with TCGA and proteomic databases. Then we constructed murine colon cancer cells over-expressing IFI35 and tested their effect on anti-tumor immunity in both immunodeficient and immunocompetent mouse models. Flow cytometry and immunohistochemistry we

SUBMITTER: Li P 

PROVIDER: S-EPMC10303345 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature