Differentiation of human induced pluripotent stem cells into functional lung alveolar epithelial cells in 3D dynamic culture.

ABSTRACT: Introduction: Understanding lung epithelium cell development from human induced pluripotent stem cells (IPSCs) in vitro can lead to an individualized model for lung engineering, therapy, and drug testing. Method: We developed a protocol to produce lung mature type I pneumocytes using encapsulation of human IPSCs in 1.1% (w/v) alginate solution within a rotating wall bioreactor system in only 20 days without using feeder cells. The aim was to reduce exposure to animal products and laborious interventions in the future. Results: The three-dimensional (3D) bioprocess allowed cell derivation into endoderm, and subsequently into type II alveolar epithelial cells within a very short period. Cells successfully expressed surfactant proteins C and B associated with type

SUBMITTER: Alsobaie S 

PROVIDER: S-EPMC10303808 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature