Rapid Identification of Lineage and Drug Resistance in Clinical Samples of Mycobacterium tuberculosis.

ABSTRACT:

Background

Mycobacterium tuberculosis is a slow-growing bacterium, which could delay its diagnosis and, therefore, promote the spread of the disease. Whole-genome sequencing allows us to obtain the complete drug-resistance profile of the strain; however, bacterial cultivation of clinical samples, along with complex processing, is required.

Methods

In this work, we explore AmpliSeq, an amplicon-based enrichment method for preparing libraries for targeted next-generation sequencing, to identify lineage and drug resistance directly from clinical samples.

Results

In our study, 111 clinical samples were tested. The lineage was identified in 100% of the culture-derived samples (52/52), in 95% of the smear (BK)-positive clinical samples (38/40) and in 42.1% of the BK-

SUBMITTER: Comin J 

PROVIDER: S-EPMC10303844 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature