Multi-neuronal recording in unrestrained animals with all acousto-optic random-access line-scanning two-photon microscopy.

ABSTRACT: To understand how neural activity encodes and coordinates behavior, it is desirable to record multi-neuronal activity in freely behaving animals. Imaging in unrestrained animals is challenging, especially for those, like larval Drosophila melanogaster, whose brains are deformed by body motion. A previously demonstrated two-photon tracking microscope recorded from individual neurons in freely crawling Drosophila larvae but faced limits in multi-neuronal recording. Here we demonstrate a new tracking microscope using acousto-optic deflectors (AODs) and an acoustic GRIN lens (TAG lens) to achieve axially resonant 2D random access scanning, sampling along arbitrarily located axial lines at a line rate of 70 kHz. With a tracking latency of 0.1 ms, this microscope recorded activitie

SUBMITTER: Yamaguchi A 

PROVIDER: S-EPMC10303936 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature