Virus Pop-Expanding Viral Databases by Protein Sequence Simulation.

ABSTRACT: The improvement of our knowledge of the virosphere, which includes unknown viruses, is a key area in virology. Metagenomics tools, which perform taxonomic assignation from high throughput sequencing datasets, are generally evaluated with datasets derived from biological samples or in silico spiked samples containing known viral sequences present in public databases, resulting in the inability to evaluate the capacity of these tools to detect novel or distant viruses. Simulating realistic evolutionary directions is therefore key to benchmark and improve these tools. Additionally, expanding current databases with realistic simulated sequences can improve the capacity of alignment-based searching strategies for finding distant viruses, which could lead to a better characterization of the "dar

SUBMITTER: Kende J 

PROVIDER: S-EPMC10304111 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature