Dual Labeling of Primary Cells with Fluorescent Gadolinium Oxide Nanoparticles.

ABSTRACT: The interest in mesenchymal stromal cells as a therapy option is increasing rapidly. To improve their implementation, location, and distribution, the properties of these must be investigated. Therefore, cells can be labeled with nanoparticles as a dual contrast agent for fluorescence and magnetic resonance imaging (MRI). In this study, a more efficient protocol for an easy synthesis of rose bengal-dextran-coated gadolinium oxide (Gd₂O₃-dex-RB) nanoparticles within only 4 h was established. Nanoparticles were characterized by zeta potential measurements, photometric measurements, fluorescence and transmission electron microscopy, and MRI. In vitro cell experiments with SK-MEL-28 and primary adipose-derived mesenchymal stromal cells (ASC), nanoparticle internalization,

SUBMITTER: Brune N 

PROVIDER: S-EPMC10304197 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature