Formononetin alleviates acute pancreatitis by reducing oxidative stress and modulating intestinal barrier.

ABSTRACT:

Background

Acute pancreatitis (AP) is a recurrent inflammatory disease. Studies have shown that intestinal homeostasis is essential for the treatment of AP. Formononetin is a plant-derived isoflavone with antioxidant properties that can effectively treat a variety of inflammatory diseases. This study aims to investigate the role of formononetin in protecting against AP and underlying mechanism.

Methods

Caerulein was used to induce AP. The inflammatory cytokines were detected using Quantitative real-time PCR and commercial kits. Histological examination was applied with hematoxylin and eosin staining. Western blot was conducted to detect expression of intestinal barrier protein and signaling molecular. Molecular docking was performed to assess protein-ligand interaction.

R

SUBMITTER: Yang J 

PROVIDER: S-EPMC10304236 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature