In Situ Structures of the Ultra-Long Extended and Contracted Tail of Myoviridae Phage P1.

ABSTRACT: The Myoviridae phage tail is a common component of contractile injection systems (CISs), essential for exerting contractile function and facilitating membrane penetration of the inner tail tube. The near-atomic resolution structures of the Myoviridae tail have been extensively studied, but the dynamic conformational changes before and after contraction and the associated molecular mechanism are still unclear. Here, we present the extended and contracted intact tail-structures of Myoviridae phage P1 by cryo-EM. The ultra-long tail of P1, 2450 Å in length, consists of a neck, a tail terminator, 53 repeated tail sheath rings, 53 repeated tube rings, and a baseplate. The sheath of the contracted tail shrinks by approximately 55%, resulting in the separation of the inner ri

SUBMITTER: Yang F 

PROVIDER: S-EPMC10304247 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature