Spatiotemporal control of genome engineering in cone photoreceptors.

ABSTRACT:

Background

Cones are essential for color recognition, high resolution, and central vision; therefore cone death causes blindness. Understanding the pathophysiology of each cell type in the retina is key to developing therapies for retinal diseases. However, studying the biology of cone cells in the rod-dominant mammalian retina is particularly challenging. In this study, we used a bacterial artificial chromosome (BAC) recombineering method to knock in the "CreER^T2" sequence into the Gnat2 and Arr3 genes, respectively and generated three novel inducible CreER^T2 mice with different cone cell specificities.

Results

These models (Gnat2^CreERT2, Arr3^T2ACreERT2, and Arr3^P2ACreERT2) express temporally controllable Cre recombinas

SUBMITTER: Wang NK 

PROVIDER: S-EPMC10304375 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature