CrebH protects against liver injury associated with colonic inflammation via modulation of exosomal miRNA.

ABSTRACT:

Background

Hepatic liver disease, including primary sclerosing cholangitis (PSC), is a serious extraintestinal manifestations of colonic inflammation. Cyclic adenosine monophosphate (cAMP)-responsive element-binding protein H (CrebH) is a transcription factor expressed mostly in the liver and small intestine. However, CrebH's roles in the gut-liver axis remain unknown.

Methods

Inflammatory bowel disease (IBD) and PSC disease models were established in wild-type and CrebH^-/- mice treated with dextran sulfate sodium, dinitrobenzene sulfonic acid, and diethoxycarbonyl dihydrocollidine diet, respectively. RNA sequencing were conducted to investigate differential gene expression. Exosomes were isolated from plasma and culture media. miRNA expression profiling was perfo

SUBMITTER: Lee SH 

PROVIDER: S-EPMC10304376 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature