On-Slide Lambda Protein Phosphatase-Mediated Dephosphorylation of Fixed Samples.

ABSTRACT: Protein phosphorylation is a ubiquitous post-translational modification that regulates a plethora of intracellular processes, making its analysis crucial for understanding intracellular dynamics. The commonly used methods, such as radioactive labeling and gel electrophoresis, do not provide information about subcellular localization. Immunofluorescence using phospho-specific antibodies and subsequent analysis via microscopy allows researchers to assess subcellular localization, but it typically lacks validation whether the observed fluorescent signal is phosphorylation specific. In this study, an on-slide dephosphorylation assay coupled with immunofluorescence staining using phospho-specific antibodies on fixed samples is proposed as a fast and simple approach to validate phosphorylated pr

SUBMITTER: Tishchenko A 

PROVIDER: S-EPMC10304472 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature