Full Copper Resistance in Cupriavidus metallidurans Requires the Interplay of Many Resistance Systems.

ABSTRACT: The metal-resistant bacterium Cupriavidus metallidurans uses its copper resistance components to survive the synergistic toxicity of copper ions and gold complexes in auriferous soils. The cup, cop, cus, and gig determinants encode as central component the Cu(I)-exporting P_IB1-type ATPase CupA, the periplasmic Cu(I)-oxidase CopA, the transenvelope efflux system CusCBA, and the Gig system with unknown function, respectively. The interplay of these systems with each other and with glutathione (GSH) was analyzed. Copper resistance in single and multiple mutants up to the quintuple mutant was characterized in dose-response curves, Live/Dead-staining, and atomic copper and glutathione content of the cells. The regulation of the cus and gig de

SUBMITTER: Hirth N 

PROVIDER: S-EPMC10304878 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature