Rapid Detection of DNA and RNA Shrimp Viruses Using CRISPR-Based Diagnostics.

ABSTRACT: Timely detection of persistent and emerging pathogens is critical to controlling disease spread, particularly in high-density populations with increased contact between individuals and limited-to-no ability to quarantine. Standard molecular diagnostic tests for surveying pathogenic microbes have provided the sensitivity needed for early detection, but lag in time-to-result leading to delayed action. On-site diagnostics alleviate this lag, but current technologies are less sensitive and adaptable than lab-based molecular methods. Towards the development of improved on-site diagnostics, we demonstrated the adaptability of a loop-mediated isothermal amplification-CRISPR coupled technology for detecting DNA and RNA viruses that have greatly impacted shrimp populations worldwide; White Spot Syn

SUBMITTER: Major SR 

PROVIDER: S-EPMC10304985 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature