Medium- and time-related effects on hypothermic storage of rat testicular cells.

ABSTRACT:

Objective

Testicular samples obtained for fertility preservation often need to be transported between clinics. This study aimed to mimic this short-term hypothermic storage (4-8 °C) and explore the impact of these conditions and the transport medium composition on prepubertal rat testicular tissue samples.

Methods

Testicular tissue samples obtained from seven days post-partum rats were transferred to six compositionally different basal culture media and a balanced salt solution, which had been kept at 4-8 °C prior to transfer. The samples were preserved for either 12 or 24 hours in these hypothermic conditions. The potential effects of the short-term storage were evaluated by assessing the morphology, measuring the testosterone levels by radioimmunoassay and analysing 96 gen

SUBMITTER: Elfageih R 

PROVIDER: S-EPMC10305459 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature