Structural Model for Factor X Inhibition of IgM and Complement-Mediated Neutralization of Adenovirus.

ABSTRACT: Adenovirus has strong therapeutic potential as an oncolytic virus and gene therapy vector. However, injecting human species C serotype 5 adenovirus, HAdv-C5, into the bloodstream leads to numerous interactions with plasma proteins that affect viral tropism and biodistribution, and can lead to potent immune responses and viral neutralization. The HAdv/factor X (FX) interaction facilitates highly efficient liver transduction and protects virus particles from complement-mediated neutralization after intravenous delivery. Ablating the FX interaction site on the HAdv-C5 capsid leaves the virus susceptible to neutralization by natural IgM followed by activation of the complement cascade and covalent binding of complement components C4b and C3b to the viral capsid. Here we present structural mode

SUBMITTER: Wagner N 

PROVIDER: S-EPMC10305487 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature