The impact of percutaneous epididymal sperm aspiration on sperm quality in mice.

ABSTRACT:

Abstract

In laboratory mice, sperm quality is usually assessed in spermatozoa collected from the cauda epididymidis of freshly sacrificed males. Percutaneous epididymal sperm aspiration (PESA) is a non-terminal alternative that would allow repeated sperm collection for sperm quality assessment in living males. To test whether PESA is a suitable method to assess sperm quality, we compared sperm traits between samples collected by PESA vs the commonly applied terminal cauda epididymidis dissection. The collected sperm samples were analyzed using computer-assisted sperm analysis and various parameters, including sperm motility, swimming velocity and morphology, were determined. We were able to retrieve motile sperm from all mice using PESA and the terminal cauda epididymidis dissectio

SUBMITTER: Windhofer L 

PROVIDER: S-EPMC10305637 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature