Dataset Information

Identification of Two Novel Carbapenemase-Encoding Hybrid Plasmids Harboring bla_NDM-5 and bla_KPC-2 in a Clinical ST11-KL47 Klebsiella pneumoniae.

ABSTRACT:

Background

Emergence of bla_KPC and bla_NDM co-harboring Klebsiella pneumoniae has escalated the threat of Carbapenem-resistant Klebsiella pneumoniae (CRKP) to healthcare. It remains unknown the prevalence and molecular characteristics of CRKP co-producing KPC and NDMs carbapenemases in Henan.

Methods and results

Twenty-seven CRKP strains isolated from different times were selected randomly in affiliated cancer hospital of Zhengzhou University from January 2019 to January 2021, among which one KPC-2 and NDM-5 positive CRKP named K9 was isolated from an abdominal pus sample of a 63-year-old male patient with leukemia. Sequencing of K9 determined that K9 belonged to ST11-KL47, which is resistant to antibiotics such as meropenem, ceftazidime-avibactam and tetracycline. K9 carried two different plasmids that contained bla_NDM-5 and bla_KPC-2. Both plasmids were shown to be novel hybrid plasmids and IS26 played an important role in generation of two plasmids. Gene bla_KPC-2 was flanked by the NTEKPC-Ib-like genetic structure (IS26-ΔTn3-ISKpn8-bla_KPC-2-ISKpn6-IS26) and was located on a conjugative IncFII/R/N type hybrid plasmid.

Conclusion

The resistance gene bla_NDM-5 located on a region organized as IS26-bla_NDM-5-ble-trpF-dsbD-ISCR1-sul1-aadA2-dfrA12-IntI1-IS26 was carried by a phage-plasmid. We described a clinical CRKP co-producing KPC-2 and NDM-5 and emphasized an urgent need to control their further spread.

SUBMITTER: Xiao W

PROVIDER: S-EPMC10305773 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Publications

Identification of Two Novel Carbapenemase-Encoding Hybrid Plasmids Harboring blaNDM-5 and blaKPC-2 in a Clinical ST11-KL47 Klebsiella pneumoniae.

Xiao Weiqiang W Wang Xiaokun X Qu Yuanye Y Sun Mingyue M Chang Yanmin Y Li Wenjiao W Shen Yong Y Shi Xiufang X Jing Min M Xu Qingxia Q

Infection and drug resistance 20230624

<h4>Background</h4>Emergence of blaKPC and blaNDM co-harboring Klebsiella pneumoniae has escalated the threat of Carbapenem-resistant Klebsiella pneumoniae (CRKP) to healthcare. It remains unknown the prevalence and molecular characteristics of CRKP co-producing KPC and NDMs carbapenemases in Henan.<h4>Methods and results</h4>Twenty-seven CRKP strains isolated from different times were selected randomly in affiliated cancer hospital of Zhengzhou ...[more]

PMID: 37388189

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