Scipion Flexibility Hub: an integrative framework for advanced analysis of conformational heterogeneity in cryoEM.

ABSTRACT: Understanding how structure and function meet to drive biological processes is progressively shifting the cryoEM field towards a more advanced analysis of macromolecular flexibility. Thanks to techniques such as single-particle analysis and electron tomography, it is possible to image a macromolecule in different states, information that can subsequently be extracted through advanced image-processing methods to build a richer approximation of a conformational landscape. However, the interoperability of all of these algorithms remains a challenging task that is left to users, preventing them from defining a single flexible workflow in which conformational information can be addressed by different algorithms. Therefore, in this work, a new framework integrated into Scipion is proposed called

SUBMITTER: Herreros D 

PROVIDER: S-EPMC10306064 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature