Chemiluminescent duplex analysis using phenoxy-1,2-dioxetane luminophores with color modulation.

ABSTRACT: Multiplex technology is an important emerging field, in diagnostic sciences, that enables the simultaneous detection of several analytes in a single sample. The light-emission spectrum of a chemiluminescent phenoxy-dioxetane luminophore can be accurately predicted by determining the fluorescence-emission spectrum of its corresponding benzoate species, which is generated during the chemiexcitation process. Based on this observation, we designed a library of chemiluminescent dioxetane luminophores with multicolor emission wavelengths. Two dioxetane luminophores that have different emission spectra, but similar quantum yield properties, were selected from the synthesized library for a duplex analysis. The selected dioxetane luminophores were equipped with two different enzymatic substrates to

SUBMITTER: Gutkin S 

PROVIDER: S-EPMC10306105 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature