The biosynthetic-secretory pathway, supplemented by recycling routes, determines epithelial membrane polarity.

ABSTRACT: In prevailing epithelial polarity models, membrane-based polarity cues (e.g., the partitioning-defective PARs) position apicobasal cellular membrane domains. Intracellular vesicular trafficking expands these domains by sorting polarized cargo toward them. How the polarity cues themselves are polarized in epithelia and how sorting confers long-range apicobasal directionality to vesicles is still unclear. Here, a systems-based approach using two-tiered C. elegans genomics-genetics screens identifies trafficking molecules that are not implicated in apical sorting yet polarize apical membrane and PAR complex components. Live tracking of polarized membrane biogenesis indicates that the biosynthetic-secretory pathway, linked to recycling routes, is asymmetrically oriented toward the apica

SUBMITTER: Zhang N 

PROVIDER: S-EPMC10306302 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature