NGS Technology in Monitoring the Genetic Diversity of Cytomegalovirus Strains.

ABSTRACT: Modern molecular genetic methods, massive parallel sequencing in particular, allow for genotyping of various pathogens with the aim of their epidemiological marking and improvement of molecular epidemiological surveillance of actual infections, including cytomegalovirus infection. The aim of the study is to evaluate the next-generation sequencing (NGS) technology for genotyping clinical isolates of cytomegalovirus (CMV).

Materials and methods

The object of the study were samples of biological substrates (leukocyte mass, saliva, urine) taken from patients who underwent liver and kidney transplantation. Detection of CMV DNA was carried out by a real-time PCR using commercial diagnostic AmpliSense CMV-FL test systems (Central Research Institute for Epidemiology, Moscow, Russia).

SUBMITTER: Vankova OE 

PROVIDER: S-EPMC10306971 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature