Lactation-associated macrophages exist in murine mammary tissue and human milk.

ABSTRACT: Macrophages are involved in immune defense, organogenesis and tissue homeostasis. Macrophages contribute to the different phases of mammary gland remodeling during development, pregnancy and involution postlactation. Less is known about the dynamics of mammary gland macrophages in the lactation stage. Here, we describe a macrophage population present during lactation in mice. By multiparameter flow cytometry and single-cell RNA sequencing, we identified a lactation-induced CD11c⁺CX3CR1⁺Dectin-1⁺ macrophage population (liMac) that was distinct from the two resident F4/80^hi and F4/80^lo macrophage subsets present pregestationally. LiMacs were predominantly monocyte-derived and expanded by proliferation in situ concomitant with nursing. Li

SUBMITTER: Cansever D 

PROVIDER: S-EPMC10307629 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature