Single-cell quantification of ribosome occupancy in early mouse development.

ABSTRACT: Translation regulation is critical for early mammalian embryonic development¹. However, previous studies had been restricted to bulk measurements², precluding precise determination of translation regulation including allele-specific analyses. Here, to address this challenge, we developed a novel microfluidic isotachophoresis (ITP) approach, named RIBOsome profiling via ITP (Ribo-ITP), and characterized translation in single oocytes and embryos during early mouse development. We identified differential translation efficiency as a key mechanism regulating genes involved in centrosome organization and N⁶-methyladenosine modification of RNAs. Our high-coverage measurements enabled, to our knowledge, the first analysis of allele-specific ribosome engagement in e

SUBMITTER: Ozadam H 

PROVIDER: S-EPMC10307641 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature