¹H-detected characterization of carbon-carbon networks in highly flexible protonated biomolecules using MAS NMR.

ABSTRACT: In the last three decades, the scope of solid-state NMR has expanded to exploring complex biomolecules, from large protein assemblies to intact cells at atomic-level resolution. This diversity in macromolecules frequently features highly flexible components whose insoluble environment precludes the use of solution NMR to study their structure and interactions. While High-resolution Magic-Angle Spinning (HR-MAS) probes offer the capacity for gradient-based ¹H-detected spectroscopy in solids, such probes are not commonly used for routine MAS NMR experiments. As a result, most exploration of the flexible regime entails either ¹³C-detected experiments, the use of partially perdeuterated systems, or ultra-fast MAS. Here we explore proton-detected pulse schemes probing thro

SUBMITTER: Bahri S 

PROVIDER: S-EPMC10307723 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature