Narcolepsy type I-associated DNA methylation and gene expression changes in the human leukocyte antigen region.

ABSTRACT: Narcolepsy type 1 (NT1) is caused by a loss of hypothalamic orexin-producing cells, and autoreactive CD4⁺ and CD8⁺ T cells have been suggested to play a role in the autoimmune mechanism. Although NT1 showed a strong association with human leukocyte antigen (HLA)-DQB1*06:02, the responsible antigens remain unidentified. We analyzed array-based DNA methylation and gene expression data for the HLA region in CD4⁺ and CD8⁺ T cells that were separated from the peripheral blood mononuclear cells of Japanese subjects (NT1, N = 42; control, N = 42). As the large number of SNPs in the HLA region might interfere with the affinity of the array probes, we conducted a comprehensive assessment of the reliability of each probe. The criteria were based on a previ

SUBMITTER: Yoshida-Tanaka K 

PROVIDER: S-EPMC10307834 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature