Development of a human malaria-on-a-chip disease model for drug efficacy and off-target toxicity evaluation.

ABSTRACT: A functional, multi-organ, serum-free system was developed for the culture of P. falciparum in an attempt to establish innovative platforms for therapeutic drug development. It contains 4 human organ constructs including hepatocytes, splenocytes, endothelial cells, as well as recirculating red blood cells which allow for infection with the parasite. Two strains of P. falciparum were used: the 3D7 strain, which is sensitive to chloroquine; and the W2 strain, which is resistant to chloroquine. The maintenance of functional cells was successfully demonstrated both in healthy and diseased conditions for 7 days in the recirculating microfluidic model. To demonstrate an effective platform for therapeutic development, systems infected with the 3D7 strain were treated with chloroquine which signif

SUBMITTER: Rupar MJ 

PROVIDER: S-EPMC10307889 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature