Cell shape characterization, alignment, and comparison using FlowShape.

ABSTRACT:

Motivation

The shape of a cell is tightly controlled, and reflects important processes including actomyosin activity, adhesion properties, cell differentiation, and polarization. Hence, it is informative to link cell shape to genetic and other perturbations. However, most currently used cell shape descriptors capture only simple geometric features such as volume and sphericity. We propose FlowShape, a new framework to study cell shapes in a complete and generic way.

Results

In our framework a cell shape is represented by measuring the curvature of the shape and mapping it onto a sphere in a conformal manner. This single function on the sphere is next approximated by a series expansion: the spherical harmonics decomposition. The decomposition facilitates many analyses, includ

SUBMITTER: van Bavel C 

PROVIDER: S-EPMC10307944 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature