Selection of Molecules with Immunological Potential from Excretory and Secretory Products from the Nematode Haemonchus placei by Cell Proliferation and Gene Expression Assays.

ABSTRACT: The nematodeHaemonchus placeiis a pathogenic parasite, the most seriously affecting ruminant's health and being responsible for enormous economic losses all over the world. The present protocol describes different in vitro techniques to select potential candidate antigens with immune-protective activity from excretory and secretory products (ESP) fromH. placeitransitory infective larvae (xL₃). ESP from xL₃were obtained from the in vitro infective larvae (L₃) maintained in Hank's medium at 37 °C with 5% CO₂for 48 h. Then, the presence of ESP proteins was confirmed by SDS-PAGE to be used in an in vitro proliferation assay with bovine peripheral blood mononuclear cells (PBMCs). The ESP were exposed to the PBMCs during two different per

SUBMITTER: Maza-Lopez J 

PROVIDER: S-EPMC10308192 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature