A Single-Entity Method for Actively Controlled Nucleation and High-Quality Protein Crystal Synthesis.

ABSTRACT: Lack of controls and understanding in nucleation, which proceeds crystal growth and other phase transitions, has been a bottleneck challenge in chemistry, materials, biology, and other fields. The exemplary needs for better methods for biomacromolecule crystallization include (1) synthesizing crystals for high-resolution structure determinations in fundamental research and (2) tuning the crystal habit and thus the corresponding properties in materials and pharmaceutical applications. Herein, a deterministic method is established capable of sustaining the nucleation and growth of a single crystal using the protein lysozyme as a prototype. The supersaturation is localized at the interface between a sample and a precipitant solution, spatially confined by the tip of a single nanopipette. The

SUBMITTER: Yang R 

PROVIDER: S-EPMC10308327 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature