Accelerating Gut Microbiome Research with Robust Sample Collection.

ABSTRACT:

Background

Inferior quality of biological material compromises data, slows discovery, and wastes research funds. The gut microbiome plays a critical role in human health and disease, yet little attention has been given to optimizing collection and processing methods of human stool.

Methods

We collected the entire bowel movement from 2 healthy volunteers: one to examine stool sample heterogeneity and one to test stool sample handling parameters. Sequencing and bioinformatic analyses were used to examine the microbiome composition.

Results

The microbiome profile varied depending on where the subsample was obtained from the stool. The exterior cortex of the stool was rich in specific phyla and deficient in others while the interior core of the stool revealed opposite mic

SUBMITTER: Zreloff ZJ 

PROVIDER: S-EPMC10308701 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature