Dataset Information

Exploration of the interplay between spatially distinct microbial habitats through comparative analysis.

ABSTRACT:

Objectives

The oral microbiome is closely associated with systemic diseases, indicating the presence of bacteremia and inflammatory mediators in the systemic circulation. Our research aims to investigate the relationship between the oral microbiome and other microbial habitats.

Methods

We analyzed 180 specimens from 36 patients, including saliva, buccal swab, plaque, stool, and blood samples from a healthy group (Non_PD, n = 18) and a periodontitis group (PD, n = 18). The final analysis included 147 specimens, with varying sample sizes for each group. Metagenomic analysis was performed using prokaryotic 16S rRNA on the MiSeq platform (Illumina).

Results

PD saliva showed significant richness differences (P's < 0.05), similar to plaque. Buccal swabs had slight variations. Microbial network analysis revealed altered microbial interactions in the PD group, with decreased interactions in saliva and buccal swabs, and increased interactions in plaque. In our analysis of nine specimens where all paired habitat samples could be analyzed, microorganisms linked to oral periodontitis were found in sterile blood samples, resembling the oral cavity's composition.

Conclusions

Microbiome differences should consider overall microbial-environment interactions, alongside diversity and richness. Our data cautiously suggest that disease-related changes in the salivary microbiome may be reflected in blood specimens through the oral-blood axis.

SUBMITTER: Kim H

PROVIDER: S-EPMC10308874 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature

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Publications

Exploration of the interplay between spatially distinct microbial habitats through comparative analysis.

Kim Hyunji H Hong Jin-Sil JS Yun Pil-Young PY Hwang Kyung-Gyun KG Kim Keun-Suh KS Lee Hyo-Jung HJ Park Kyoung Un KU

Journal of oral microbiology 20230627 1

<h4>Objectives</h4>The oral microbiome is closely associated with systemic diseases, indicating the presence of bacteremia and inflammatory mediators in the systemic circulation. Our research aims to investigate the relationship between the oral microbiome and other microbial habitats.<h4>Methods</h4>We analyzed 180 specimens from 36 patients, including saliva, buccal swab, plaque, stool, and blood samples from a healthy group (Non_PD, <i>n</i> = 18) and a periodontitis group (PD, <i>n</i> = 18) ...[more]

PMID: 37396300

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Dataset Information

Exploration of the interplay between spatially distinct microbial habitats through comparative analysis.

Objectives

Methods

Results

Conclusions

Publications

Exploration of the interplay between spatially distinct microbial habitats through comparative analysis.

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