Targeted depletion of pks+ bacteria from a fecal microbiota using specific antibodies.

ABSTRACT: The pks island is one of the most prevalent pathogenicity islands among the Escherichia coli strains that colonize the colon of colorectal carcinoma (CRC) patients. This pathogenic island encodes the production of a nonribosomal polyketide-peptide named colibactin, which induces double-strand breaks in DNA molecules. Detection or even depletion of this pks-producing bacteria could help to understand the role of these strains in the context of CRC. In this work, we performed a large-scale in silico screening of the pks cluster in more than 6,000 isolates of E. coli. The results obtained reveal that not all the pks-detected strains could produce a functional genotoxin and, using antibodies against pks-specific peptides from surface cell

SUBMITTER: Blanco-Miguez A 

PROVIDER: S-EPMC10308883 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature