Conserved Characteristics of NMPylation Activities of Alpha- and Betacoronavirus NiRAN Domains.

ABSTRACT: Coronavirus genome replication and expression are mediated by the viral replication-transcription complex (RTC) which is assembled from multiple nonstructural proteins (nsp). Among these, nsp12 represents the central functional subunit. It harbors the RNA-directed RNA polymerase (RdRp) domain and contains, at its N terminus, an additional domain called NiRAN which is widely conserved in coronaviruses and other nidoviruses. In this study, we produced bacterially expressed coronavirus nsp12s to investigate and compare NiRAN-mediated NMPylation activities from representative alpha- and betacoronaviruses. We found that the four coronavirus NiRAN domains characterized to date have a number of conserved properties, including (i) robust nsp9-specific NMPylation activities that appear to operate l

SUBMITTER: Slanina H 

PROVIDER: S-EPMC10308930 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature